- 品名： 普諾賽Daoy (人髓母細(xì)胞瘤細(xì)胞)
- 型號(hào)： CL-0615
- 產(chǎn)品詳情
- 規(guī)格參數(shù)
產(chǎn)品信息
| 名稱 | Daoy (人髓母細(xì)胞瘤細(xì)胞) (STR鑒定正確) |
| 別稱 | DAOY; D324 Med; D-324 Med; D324 MED; D-324MED; D324 |
| 背景描述 | The Daoy cell line was established in 1985 by P. F Jacobsen of the Royal Perth Hospital in Western Australia. The line was derived from biopsy material taken from a tumor in the posterior fossa of a 4 year old boy. |
| 種屬 | 人 |
| 年齡（性別） | 男；4歲 |
| 組織來源 | 結(jié)締組織增生性小腦髓母細(xì)胞 |
| 生長(zhǎng)特性 | 貼壁細(xì)胞 |
| 細(xì)胞形態(tài) | 上皮細(xì)胞樣 |
| 細(xì)胞類型 | 腫瘤細(xì)胞 |
| 腫瘤類型 | 神經(jīng)母細(xì)胞瘤細(xì)胞 |
| 生物安全等級(jí) | 1 |
| 生長(zhǎng)培養(yǎng)基 | MEM（含NEAA）＋10% FBS＋1%P/S |
| 推薦傳代比例 | 1:3-1:6 |
| 推薦換液頻率 | 2~3次/周 |
| 倍增時(shí)間 | 33.6 hours, at 48th passage (PubMed=2993532); 34 hours (ATCC) |
| 凍存條件 | 凍存液：55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO溫度：液氮 |
| 培養(yǎng)條件 | 氣相：空氣，95%；CO2，5%溫度：37℃ |
| 致瘤性 | Yes, in nude mice(The cells form serially transplantable intercranial and subcutaneous tumors.) |
| 保藏機(jī)構(gòu) | ATCC; HTB-186 BCRJ; 0325 |
STR鑒定
STR位點(diǎn)信息
Amelogenin X CSF1PO 11 D2S1338 16,17 D3S1358 15 D5S818 11,13 D7S820 8,10 D8S1179 13,15 D13S317 13,14 D16S539 10 D18S51 12 D19S433 14.2 D21S11 29,31.2 FGA 23 PentaD 10,13 PentaE 7,11 TH01 9 TPOX 8,10 vWA 14,20 D6S1043 12 D12S391 20 D2S441 10,11
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